(Peer-Reviewed) Label-free trace detection of bio-molecules by liquid-interface assisted surface-enhanced Raman scattering using a microfluidic chip
Shi Bai ¹, Xueli Ren ², Kotaro Obata ¹, Yoshihiro Ito ², Koji Sugioka ¹
¹ Advanced Laser Processing Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
² Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Opto-Electronic Advances, 2022-08-20
Abstract
Surface-enhanced Raman scattering (SERS), owing to its high sensitivity based on localized surface plasmon resonance of nanostructured metals, is recently attracting much attention to be used for biotechnology, such as cell imaging and tumor therapy. On the other hand, the trace detection of bio-molecules with large molecular weight is still challenging because the troublesome treatment of SERS substrate using coupling or cross-linking agents is required.
In this paper, we apply liquid interface assisted SERS (LI-SERS) method, which provides unique features of collection and self-immobilization of analyte molecules on the SERS substrate, to realize the label-free trace detection of bio-molecules with detection limits of pM ~ fM. Specifically, deoxyribonucleic acid (DNA) discrimination and quantitative detection of β-Amyloid (Aβ) in trace-concentration are demonstrated to illustrate the ultrahigh sensitivity and versatility of the LI-SERS method.
The results suggest LI-SERS is promising for the early-stage diagnosis of diseases such as virus infection and Alzheimer's disease.
Switching of K-Q intervalley trions fine structure and their dynamics in n-doped monolayer WS₂
Jiajie Pei, Xue Liu, Andrés Granados del Águila, Di Bao, Sheng Liu, Mohamed-Raouf AMARA, Weijie Zhao, Feng Zhang, Congya You, Yongzhe Zhang, Kenji Watanabe, Takashi Taniguchi, Han Zhang, Qihua Xiong
Opto-Electronic Advances
2022-10-28
Large-scale and high-quality III-nitride membranes through microcavity-assisted crack propagation by engineering tensile-stressed Ni layers
Jung-Hong Min, Kwangjae Lee, Tae-Hoon Chung, Jung-Wook Min, Kuang-Hui L1, Chun Hong Kang, Hoe-Min Kwak, Tae-Hyeon Kim, Youyou Yuan, Kyoung-Kook Kim, Dong-Seon Lee, Tien Khee Ng, Boon S. Ooi
Opto-Electronic Science
2022-10-28
Crosstalk-free achromatic full Stokes imaging polarimetry metasurface enabled by polarization-dependent phase optimization
Yaxin Zhang, Mingbo Pu, Jinjin Jin1, Xinjian Lu, Yinghui Guo, Jixiang Cai, Fei Zhang, Yingli Ha, Qiong He, Mingfeng Xu, Xiong Li, Xiaoliang Ma, Xiangang Luo
Opto-Electronic Advances
2022-09-30