Year
Month
(Peer-Reviewed) Minocycline Activates the Nucleus of the Solitary Tract-Associated Network to Alleviate Lipopolysaccharide-Induced Neuroinflammation
Jianbo Xiu 修建波 ¹ ², Lanlan Li 李岚岚 ¹ ², Qi Xu 许琪 ¹ ²
¹ State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China
中国 北京 中国医学科学院基础医学研究所 北京协和医学院基础学院 医学分子生物学国家重点实验室
² Neuroscience Center, Chinese Academy of Medical Sciences, Beijing 100005, China
中国 北京 中国医学科学院神经科学中心
Abstract

Objective

To examine the neuroanatomical substrates underlying the effects of minocycline in alleviating lipopolysaccharide (LPS)-induced neuroinflammation.

Methods

Forty C57BL/6 male mice were randomly and equally divided into eight groups. Over three conse-cutive days, saline was administered to four groups of mice and minocycline to the other four groups. Immediately after the administration of saline or minocycline on the third day, two groups of mice were additionally injected with saline and the other two groups were injected with LPS. Six or 24 hours after the last injection, mice were sacrificed and the brains were removed. Immunohistochemical staining across the whole brain was performed to detect microglia activation via Iba1 and neuronal activation via c-Fos. Morphology of microglia and the number of c-Fo-positive neurons were analyzed by Image-Pro Premier 3D. One-way ANOVA and Fisher’s least-significant differences were employed for statistical analyses.

Results

Minocycline alleviated LPS-induced neuroinflammation as evidenced by reduced activation of microglia in multiple brain regions, including the shell part of the nucleus accumbens (Acbs), paraventricular nucleus (PVN) of the hypothalamus, central nucleus of the amygdala (CeA), locus coeruleus (LC), and nucleus tractus solitarius (NTS). Minocycline significantly increased the number of c-Fo-positive neurons in NTS and area postrema (AP) after LPS treatment. Furthermore, in NTS-associated brain areas, including LC, lateral parabrachial nucleus (LPB), periaqueductal gray (PAG), dorsal raphe nucleus (DR), amygdala, PVN, and bed nucleus of the stria terminali (BNST), minocycline also significantly increased the number of c-Fo-positive neurons after LPS administration.

Conclusion

Minocycline alleviates LPS-induced neuroinflammation in multiple brain regions, possibly due to increased activation of neurons in the NTS-associated network.
Minocycline Activates the Nucleus of the Solitary Tract-Associated Network to Alleviate Lipopolysaccharide-Induced Neuroinflammation_1
Minocycline Activates the Nucleus of the Solitary Tract-Associated Network to Alleviate Lipopolysaccharide-Induced Neuroinflammation_2
Minocycline Activates the Nucleus of the Solitary Tract-Associated Network to Alleviate Lipopolysaccharide-Induced Neuroinflammation_3
Minocycline Activates the Nucleus of the Solitary Tract-Associated Network to Alleviate Lipopolysaccharide-Induced Neuroinflammation_4
  • Giant and light modifiable third-order optical nonlinearity in a free-standing h-BN film
  • Jun Ren, Han Lin, Xiaorui Zheng, Weiwei Lei, Dan Liu, Tianling Ren, Pu Wang4, Baohua Jia
  • Opto-Electronic Science
  • 2022-06-21
  • Research status and prospect of wind-vehicle-bridge coupling vibration system
  • Wanshui Han, Xiaodong Liu, Xuelian Guo, Shizhi Chen, Gan Yang, Huanju Liu
  • Journal of Traffic and Transportation Engineering
  • 2022-06-20
  • The reservoir learning power across quantum many-body localization transition
  • Wei Xia, Jie Zou, Xingze Qiu, Xiaopeng Li
  • Frontiers of Physics
  • 2022-06-15
  • A small microring array that performs large complex-valued matrix-vector multiplication
  • Junwei Cheng, Yuhe Zhao, Wenkai Zhang, Hailong Zhou, Dongmei Huang, Qing Zhu, Yuhao Guo, Bo Xu, Jianji Dong, Xinliang Zhang
  • Frontiers of Optoelectronics
  • 2022-06-15
  • Prognostic role of plasma levels of γ-glutamyl transpeptidase in patients with advanced gastric cancer treated with anti-PD-1 immunotherapy
  • Shaojie Xu, Yiming Feng, Xingyin Li, Zaozao Huang, Hewei Li, Ganxin Wang
  • Oncology and Translational Medicine
  • 2022-06-14
  • High-quality Development Paths of Ice-snow Tourism in China from the Perspective of the Winter Olympics
  • TANG Chengcai, ZENG Rui, YANG Yuanyuan, XU Shiyi, WANG Xin
  • Journal of Resources and Ecology
  • 2022-06-07
  • New approach for the digital reconstruction of complex mine faults and its application in mining
  • Hongwei Wang, Zeliang Wang, Yaodong Jiang, Jiaqi Song, Meina Jia
  • International Journal of Coal Science & Technology
  • 2022-06-06
  • p62/SQSTM1 Participates in the Innate Immune Response of Macrophages Against Candida albicans Infection
  • He Yan-Zhi, Duan Zhi-Min, Chen Xu, Li Min
  • International Journal of Dermatology and Venereology
  • 2022-06-01
  • Long term performance of recycled concrete beams with different water–cement ratio and recycled aggregate replacement rate
  • Jingwei YING, Feiming SU, Shuangren CHEN
  • Frontiers of Structural and Civil Engineering
  • 2022-05-31
  • Polarization-switchable plasmonic emitters based on laser-induced bubbles
  • Jianjun Chen, Fengyuan Gan
  • Opto-Electronic Advances
  • 2022-05-27
  • Broad-band spatial light modulation with dual epsilon-near-zero modes
  • Long Wen, Xianghong Nan, Jiaxiang Li, David R. S. Cumming, Xin Hu, Qin Chen
  • Opto-Electronic Advances
  • 2022-05-27
  • Configurable topological beam splitting via antichiral gyromagnetic photonic crystal
  • Jianfeng Chen, Zhi-Yuan Li
  • Opto-Electronic Science
  • 2022-05-20



  • Field distribution of the Z₂ topological edge state revealed by cathodoluminescence nanoscopy                                Cylindrical vector beams reveal radiationless anapole condition in a resonant state
    About
    |
    Contact
    |
    Copyright © PubCard