Year
Month
(Peer-Reviewed) Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis
JIN Jing-jing 金京京 ¹ ², DUAN Shuo-nan 段硕楠 ³, QI Yong-zhi 齐永志 ¹ ², ZHEN Wen-chao 甄文超 ² ⁴, MA Jun 马骏 ³
¹ College of Plant Protection, Hebei Agricultural University, Baoding 071001, P.R.China
中国 保定 河北农业大学植物保护学院
² State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071001, P.R.China
中国 保定 华北作物改良与调控国家重点实验室
³ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
中国 北京 中国农业大学农学与生物技术学院
⁴ College of Agronomy, Hebei Agricultural University, Baoding 071001, P.R.China
中国 保定 河北农业大学农学院
Abstract

Fusarium crown rot (FCR), typically caused by Fusarium pseudograminearum, is a severe soil-borne disease that, in recent years, has become an emerging threat to Chinese wheat crops. For the first time in this study, we investigated and compared the proteomic characteristics of two Chinese wheat varieties (04 Zhong 36 and Xinmai 26) at 24, 48, and 72 h post-inoculation using label-free quantitative proteomic analysis.

A total of 9 234 proteins were successfully quantified, of which 783 were differentially expressed after inoculation. These proteins were mainly involved in metabolic, single-organism, and cellular processes. Thirty-three proteins associated with defense, cell wall formation, photosynthesis, etc., showed consistently different expression between the two genotypes at multiple time points. In particular, chitinase, which degrades chitin in the fungal cell wall and limits fungal growth, was exclusively and consistently upregulated in 04 Zhong 36 across the three time points. Other proteins such as flavonoid O-methyltransferase, glycosyltransferase, and peroxidase were only upregulated in 04 Zhong 36, and proteins, including the berberine bridge enzyme and rubisco large subunit-binding protein, were specifically downregulated in Xinmai 26.

The expression of transcripts encoding eight selected proteins through qRT-PCR analysis supported the proteomic profiles. Overall, the results of this study allow us to understand FCR resistance in wheat at the protein level. Some proteins and their corresponding genes may be useful resources for the genetic improvement of FCR resistance in wheat.
Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis_1
Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis_2
Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis_3
Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis_4
  • Power grid fault diagnosis based on a deep pyramid convolutional neural network
  • Xu Zhang 张旭, Huiting Zhang, Dongying Zhang, Yixian Wang, Ruiting Ding, Yuchuan Zheng, Yongxu Zhang
  • CSEE Journal of Power and Energy Systems
  • 2022-05-06
  • China's factor reallocation effect considering energy
  • Guangqing Xu, Xiaoyu Chen
  • Chinese Journal of Population, Resources and Environment
  • 2022-05-02
  • Cannabidiol prevents depressive-like behaviors through the modulation of neural stem cell differentiation
  • Ming Hou, Suji Wang, Dandan Yu, Xinyi Lu, Xiansen Zhao, Zhangpeng Chen, Chao Yan
  • Frontiers of Medicine
  • 2022-04-26
  • Cultivation of gut microorganisms of the marine ascidian Halocynthia roretzi reveals their potential roles in the environmental adaptation of their host
  • Yang Yang, Yuting Zhu, Haiming Liu, Jiankai Wei, Haiyan Yu, Bo Dong
  • Marine Life Science & Technology
  • 2022-04-26
  • Data network traffic analysis and optimization strategy of real-time power grid dynamic monitoring system for wide-frequency measurements
  • Jinsong Li, Hao Liu, Wenzhuo Li, Tianshu Bi, Mingyang Zhao
  • Global Energy Interconnection
  • 2022-04-25
  • Field distribution of the Z₂ topological edge state revealed by cathodoluminescence nanoscopy
  • Xiao He, Donglin Liu, Hongfei Wang, Liheng Zheng, Bo Xu, Biye Xie, Meiling Jiang, Zhixin Liu, Jin Zhang, Minghui Lu, Zheyu Fang
  • Opto-Electronic Advances
  • 2022-04-25
  • Advances in femtosecond laser direct writing of fiber Bragg gratings in multicore fibers: technology, sensor and laser applications
  • Alexey Wolf, Alexander Dostovalov, Kirill Bronnikov, Mikhail Skvortsov, Stefan Wabnitz, Sergey Babin
  • Opto-Electronic Advances
  • 2022-04-25
  • Graphene-empowered dynamic metasurfaces and metadevices
  • Chao Zeng, Hua Lu, Dong Mao, Yueqing Du, He Hua, Wei Zhao, Jianlin Zhao
  • Opto-Electronic Advances
  • 2022-04-25
  • Charge carrier dynamics in different crystal phases of CH₃NH₃PbI₃ perovskite
  • Efthymis Serpetzoglou, Ioannis Konidakis, George Kourmoulakis, Ioanna Demeridou, Konstantinos Chatzimanolis, Christos Zervos, George Kioseoglou, Emmanuel Kymakis, Emmanuel Stratakis
  • Opto-Electronic Science
  • 2022-04-21
  • Applications of optically and electrically driven nanoscale bowtie antennas
  • Zhongjun Jiang, Yingjian Liu, Liang Wang
  • Opto-Electronic Science
  • 2022-04-20
  • Validation of the bodily expressive action stimulus test among Chinese adults and children
  • Yunmei Yang, Wenwen Hou, Jing Li
  • PsyCh Journal
  • 2022-04-17



  • Effect of Cu-Rich Phase Precipitation on the Microstructure and Mechanical Properties of CoCrNiCux Medium-Entropy Alloys Prepared via Laser Directed Energy Deposition                                Effect of biochar applied with plant growth-promoting rhizobacteria (PGPR) on soil microbial community composition and nitrogen utilization in tomato
    About
    |
    Contact
    |
    Copyright © PubCard