(Peer-Reviewed) Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution
Xin Xu 徐欣 ¹ ², Qin Luo 罗勤 ¹ ², Jixiang Wang 王吉祥 ¹ ², Yahui Song 宋雅慧 ¹ ², Hong Ye 叶虹 ², Xin Zhang 张欣 ², Yi He 何益 ², Minxuan Sun 孙敏轩 ¹ ², Ruobing Zhang 张若冰 ¹ ², Guohua Shi 史国华 ¹ ²
¹ School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
中国 合肥 中国科学技术大学 生命科学与医学部 生物医学工程学院（苏州）
² Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou 215163, China
中国 苏州 中国科学院 苏州生物医学工程技术研究所 江苏省医用光学重点实验室
Opto-Electronic Advances, 2024-06-11
Abstract
Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view, typically around 1 mm. This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample, such as different brain areas. In addition, conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.
Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm, a numerical aperture of 0.5, and a working wavelength range from 400 to 1000 nm. We achieved a resolution of 0.74 μm in fluorescent beads imaging. The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system, a confocal laser scanning system, and a two-photon imaging system.
This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.
Multi-photon neuron embedded bionic skin for high-precision complex texture and object reconstruction perception research
Hongyu Zhou, Chao Zhang, Hengchang Nong, Junjie Weng, Dongying Wang, Yang Yu, Jianfa Zhang, Chaofan Zhang, Jinran Yu, Zhaojian Zhang, Huan Chen, Zhenrong Zhang, Junbo Yang
Opto-Electronic Advances
2025-01-22
