(Peer-Reviewed) Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution
Xin Xu 徐欣 ¹ ², Qin Luo 罗勤 ¹ ², Jixiang Wang 王吉祥 ¹ ², Yahui Song 宋雅慧 ¹ ², Hong Ye 叶虹 ², Xin Zhang 张欣 ², Yi He 何益 ², Minxuan Sun 孙敏轩 ¹ ², Ruobing Zhang 张若冰 ¹ ², Guohua Shi 史国华 ¹ ²
¹ School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
中国 合肥 中国科学技术大学 生命科学与医学部 生物医学工程学院(苏州)
² Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou 215163, China
中国 苏州 中国科学院 苏州生物医学工程技术研究所 江苏省医用光学重点实验室
Opto-Electronic Advances, 2024-06-11
Abstract
Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view, typically around 1 mm. This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample, such as different brain areas. In addition, conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.
Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm, a numerical aperture of 0.5, and a working wavelength range from 400 to 1000 nm. We achieved a resolution of 0.74 μm in fluorescent beads imaging. The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system, a confocal laser scanning system, and a two-photon imaging system.
This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.
Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome
Yuran Huang, Zhimin Zhang, Wenli Tao, Yunfei Wei, Liang Xu, Wenwen Gong, Jiaqiang Zhou, Liangcai Cao, Yong Liu, Yubing Han, Cuifang Kuang, Xu Liu
Opto-Electronic Advances
2024-07-05
Photonics-assisted THz wireless communication enabled by wide-bandwidth packaged back-illuminated modified uni-traveling-carrier photodiode
Yuxin Tian, Boyu Dong, Yaxuan Li, Bing Xiong, Junwen Zhang, Changzheng Sun, Zhibiao Hao, Jian Wang, Lai Wang, Yanjun Han, Hongtao Li, Lin Gan, Nan Chi, Yi Luo
Opto-Electronic Science
2024-07-01