(Peer-Reviewed) A chloride efflux transporter, BIG RICE GRAIN 1, is involved in mediating grain size and salt tolerance in rice
Zhijie Ren ¹, Fenglin Bai ¹, Jingwen Xu ¹, Li Wang ¹, Xiaohan Wang ¹, Qian Zhang ¹, Changxin Feng ¹, Qi Niu ¹, Liying Zhang ¹, Jiali Song ¹, Fang Bao ¹, Liangyu Liu 刘良玉 ¹, Yikun He 何奕騉 ¹, Ligeng Ma 马力耕 ¹, Wang Tian 田望 ², Congcong Hou 侯聪聪 ¹, Legong Li 李乐攻 ¹
¹ College of Life Sciences, Capital Normal University, and Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing 100048, China
中国 北京 首都师范大学生命科学学院 植物基因资源与低碳环境生物技术北京市重点实验室
² School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China
中国 北京 北京大学现代农学院
Abstract
Grain size is determined by the size and number of cells in the grain. The regulation of grain size is crucial for improving crop yield; however, the genes and molecular mechanisms that control grain size remain elusive. Here, we report that a member of the detoxification efflux carrier /Multidrug and Toxic Compound Extrusion (DTX/MATE) family transporters, BIG RICE GRAIN 1 (BIRG1), negatively influences grain size in rice (Oryza sativa L.).
BIRG1 is highly expressed in reproductive organs and roots. In birg1 grain, the outer parenchyma layer cells of spikelet hulls are larger than in wild-type (WT) grains, but the cell number is unaltered. When expressed in Xenopus laevis oocytes, BIRG1 exhibits chloride efflux activity. Consistent with this role of BIRG1, the birg1 mutant shows reduced tolerance to salt stress at a toxic chloride level. Moreover, grains from birg1 plants contain a higher level of chloride than those of WT plants when grown under normal paddy field conditions, and the roots of birg1 accumulate more chloride than those of WT under saline conditions.
Collectively, the data suggest that BIRG1 in rice functions as a chloride efflux transporter that is involved in mediating grain size and salt tolerance by controlling chloride homeostasis.
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Opto-Electronic Advances
2025-01-22
