(Peer-Reviewed) Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice
Wang Tao 王涛, Lou Lijuan 娄丽娟, Li Zeyu, Shang Lianguang 商连光, Wang Quan 汪泉
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture/Genome Analysis Laboratory of Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
中国 深圳 中国农业科学院深圳农业基因组研究所 岭南现代农业科学与技术广东省实验室深圳分中心 农业部农业基因数据分析重点实验室
Abstract
Protein prenylation plays a crucial role in plant development and stress response. We report the function of prenyltransferase α-subunit in rice. Protein-protein interactions showed that the farnesyl- transferase (OsPFT)/geranylgeranyltransferase-I (OsPGGT I-α) protein interacted together with OsPFT-β and OsPGGT I-β. The α- and β-subunits of OsPFT formed a heterodimer for the transfer of a farnesyl group from farnesyl pyrophosphate to the CaaX-box-containing peptide N-dansyl-GCVLS.
Furthermore, the tissue expression patterns of the OsPFT and OsPGGT I subunits were similar, and these subunits were localized in the cytoplasm and nucleus. Moreover, OsPFT/OsPGGT I-α-deletion homozygous rice mutants had a lethal phenotype, and the heterozygous mutants exhibited reduced pollen viability. These results indicated that prenylation plays an important role in rice development.
Inverse design for material anisotropy and its application for a compact X-cut TFLN on-chip wavelength demultiplexer
Jiangbo Lyu, Tao Zhu, Yan Zhou, Zhenmin Chen, Yazhi Pi, Zhengtong Liu, Xiaochuan Xu, Ke Xu, Xu Ma, Lei Wang, Zizheng Cao, Shaohua Yu
Opto-Electronic Science
2024-01-09
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Xinyu Wang, Xuke Qiu, Mulong Liu, Feng Liu, Mengmeng Li, Linpei Xue, Bohan Chen, Mingran Zhang, Peng Xie
Opto-Electronic Science
2023-12-29
