Year
Month

(Peer-Reviewed) 3D imaging lipidometry in single cell by in-flow holographic tomography
Daniele Pirone ¹ ², Daniele Sirico ¹ ³, Lisa Miccio ¹, Vittorio Bianco ¹, Martina Mugnano ¹, Danila del Giudice ¹ ⁴, Gianandrea Pasquinelli ⁵ ⁶, Sabrina Valente ⁵, Silvia Lemma ⁷ ⁸ ⁹, Luisa Iommarini ⁹ ¹⁰, Ivana Kurelac ⁷ ⁸ ⁹, Pasquale Memmolo ¹, Pietro Ferraro ¹
¹ CNR-ISASI, Institute of Applied Sciences and Intelligent Systems “E. Caianiello”, Via Campi Flegrei 34, Pozzuoli, Napoli 80078, Italy
² Department of Electrical Engineering and Information Technologies, University of Naples “Federico II”, via Claudio 21, Napoli 80125, Italy
³ Department of Chemical, Materials and Production Engineering of the University of Naples Federico II, Piazzale Tecchio 80, Napoli 80125, Italy
⁴ Department of Mathematics and Physics, University of Campania “Luigi Vanvitelli”, Caserta 81100, Italy
⁵ Biotechnology and Methods in Laboratory Medicine, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Bologna 40126, Italy
⁶ Subcellular Nephro-Vascular Diagnostic Program, Pathology Unit S.Orsola IRCCS, University of Bologna, Bologna 40126, Italy
⁷ Unit of Medical Genetics, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Via Massarenti 9, Bologna 40138, Italy
⁸ Study and Research Center on Gynecological Neoplasias, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Via Massarenti 9, Bologna 40138, Italy
⁹ Center for Applied Biomedical Research (CRBA), University of Bologna, Bologna 40138, Italy
¹⁰ Department of Pharmacy and Biotechnology (FABIT), University of Bologna, Bologna 40126, Italy
Opto-Electronic Advances , 2022-08-25
Abstract

The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets (LDs) in several regulatory mechanisms in living cells. LDs are dynamic organelles and therefore their complete characterization in terms of number, size, spatial positioning and relative distribution in the cell volume can shed light on the roles played by LDs.

Until now, fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and specificity. However, such methods generally only provide 2D assays and partial measurements. Furthermore, both can be destructive and with low productivity, thus limiting analysis of large cell numbers in a sample.

Here we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer, by using ovarian cancer cells and monocyte cell lines as models. A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is reported.

The information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.
3D imaging lipidometry in single cell by in-flow holographic tomography_1
3D imaging lipidometry in single cell by in-flow holographic tomography_2
3D imaging lipidometry in single cell by in-flow holographic tomography_3
3D imaging lipidometry in single cell by in-flow holographic tomography_4
  • Review for wireless communication technology based on digital encoding metasurfaces
  • Haojie Zhan, Manna Gu, Ying Tian, Huizhen Feng, Mingmin Zhu, Haomiao Zhou, Yongxing Jin, Ying Tang, Chenxia Li, Bo Fang, Zhi Hong, Xufeng Jing, Le Wang
  • Opto-Electronic Advances
  • 2025-07-17
  • Coulomb attraction driven spontaneous molecule-hotspot paring enables universal, fast, and large-scale uniform single-molecule Raman spectroscopy
  • Lihong Hong, Haiyao Yang, Jianzhi Zhang, Zihan Gao, Zhi-Yuan Li
  • Opto-Electronic Advances
  • 2025-07-17
  • Multiphoton intravital microscopy in small animals of long-term mitochondrial dynamics based on super‐resolution radial fluctuations
  • Saeed Bohlooli Darian, Jeongmin Oh, Bjorn Paulson, Minju Cho, Globinna Kim, Eunyoung Tak, Inki Kim, Chan-Gi Pack, Jung-Man Namgoong, In-Jeoung Baek, Jun Ki Kim
  • Opto-Electronic Advances
  • 2025-07-17
  • Research progress on generating perfect vortex beams based on metasurfaces
  • Xiujuan Liu, Manna Gu, Ying Tian, Mingfeng Zheng, Bo Fang, Zhi Hong, Chee Leong Tan, Xufeng Jing
  • Opto-Electronic Science
  • 2025-07-09
  • Non-volatile tunable multispectral compatible infrared camouflage based on the infrared radiation characteristics of Rosaceae plants
  • Xin Li, Xinye Liao, Junxiang Zeng, Zao Yi, Xin He, Jiagui Wu, Huan Chen, Zhaojian Zhang, Yang Yu, Zhengfu Zhang, Sha Huang, Junbo Yang
  • Opto-Electronic Advances
  • 2025-07-09
  • Spectro-polarimetric detection enabled by multidimensional metasurface with quasi-bound states in the continuum
  • Haoyang He, Fangxing Lai, Yan Zhang, Xue Zhang, Chenyi Tian, Xin Li, Yongtian Wang, Shumin Xiao, Lingling Huang
  • Opto-Electronic Advances
  • 2025-06-30
  • Emerging low-dimensional perovskite resistive switching memristors: from fundamentals to devices
  • Shuanglong Wang, Hong Lian, Haifeng Ling, Hao Wu, Tianxiao Xiao, Yijia Huang, Peter Müller-Buschbaum
  • Opto-Electronic Advances
  • 2025-06-27
  • CW laser damage of ceramics induced by air filament
  • Chuan Guo, Kai Li, Zelin Liu, Yuyang Chen, Junyang Xu, Zhou Li, Wenda Cui, Changqing Song, Cong Wang, Xianshi Jia, Ji'an Duan, Kai Han
  • Opto-Electronic Advances
  • 2025-06-27
  • High fiber-to-fiber net gain in erbium-doped thin film lithium niobate waveguide amplifier as an external gain chip
  • Jinli Han, Mengqi Li, Rongbo Wu, Jianping Yu, Lang Gao, Zhiwei Fang, Min Wang, Youting Liang, Haisu Zhang, Ya Cheng
  • Opto-Electronic Science
  • 2025-06-26
  • Eco-friendly quantum-dot light-emitting diode display technologies: prospects and challenges
  • Gao Peili, Li Chan, Zhou Hao, He Songhua, Yin Zhen, Ng Kar Wei, Wang Shuangpeng
  • Opto-Electronic Science
  • 2025-06-25
  • Operando monitoring of state of health for lithium battery via fiber optic ultrasound imaging system
  • Chen Geng, Wang Anqi, Zhang Yi, Zhang Fujun, Xu Dongchen, Liu Yueqi, Zhang Zhi, Yan Zhijun, Li Zhen, Li Hao, Sun Qizhen
  • Opto-Electronic Science
  • 2025-06-25
  • Observation of polaronic state assisted sub-bandgap saturable absorption
  • Li Zhou, Yiduo Wang, Jianlong Kang, Xin Li, Quan Long, Xianming Zhong, Zhihui Chen, Chuanjia Tong, Keqiang Chen, Zi-Lan Deng, Zhengwei Zhang, Chuan-Cun Shu, Yongbo Yuan, Xiang Ni, Si Xiao, Xiangping Li, Yingwei Wang, Jun He
  • Opto-Electronic Advances
  • 2025-06-19



  • Learning-based joint UAV trajectory and power allocation optimization for secure IoT networks        Carnivorous plants inspired shape-morphing slippery surfaces
    About
    |
    Contact
    |
    Copyright © PubCard