Year
Month
(Peer-Reviewed) Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics
Suwei Gao 高素伟 ¹ ² ³, Qiang Shi ⁴, Yifan Zhang 张一帆 ¹ ² ³, Guixian Liang 梁桂仙 ¹ ² ³, Zhixin Kang 康志鑫 ¹ ² ³, Baofeng Huang 黄宝凤 ¹ ² ³, Dongyuan Ma 马东媛 ¹ ², Lu Wang 王璐 ⁵, Jianwei Jiao 焦建伟 ² ³ ⁶, Xiangdong Fang ² ³ ⁷ ⁸, Cheng-Ran Xu ⁹ ¹⁰, Longqi Liu ¹¹ ¹², Xun Xu 徐讯 ¹¹ ¹³, Berthold Göttgens ¹⁴, Cheng Li 李程 ⁴, Feng Liu 刘峰 ¹ ² ³
¹ State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China 中国科学院 动物研究所 膜生物学国家重点实验室
² Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China 中国科学院 干细胞与再生医学创新研究院
³ University of Chinese Academy of Sciences, Beijing, China 中国科学院大学
⁴ School of Life Sciences, Center for Bioinformatics, Center for Statistical Science, Peking University, Beijing, China 北京大学 生命科学学院 统计科学中心 生物信息中心
⁵ State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China 中国医学科学院 北京协和医学院 血液学研究所 实验血液学国家重点实验室
⁶ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China 中国科学院 动物研究所 干细胞与生殖生物学国家重点实验室
⁷ CAS Key Laboratory of Genome Science & Information, Beijing Institute of Genomics, Chinese Academy of Sciences/China National Center for Bioinformation, Beijing, China 中国科学院 北京基因组研究所 国家生物信息中心 基因组科学与信息重点实验室
⁸ Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing, China 基因组与精准医学检测技术北京市重点实验室
⁹ Department of Human Anatomy, Histology, and Embryology, School of Basic Medical Sciences, Peking University, Beijing, China 北京大学 基础医学院 人体解剖与组织胚胎学系
¹⁰ Peking–Tsinghua Center for Life Sciences, Peking University, Beijing, China 清华大学-北京大学生命科学联合中心
¹¹ BGI-ShenZhen, Shenzhen, Guangdong, China 深圳华大生命科学研究院
¹² Shenzhen Bay Laboratory, Shenzhen, Guangdong, China 深圳湾实验室
¹³ Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen, Guangdong, China 广东省高通量基因组测序与合成编辑重点实验室
¹⁴ Department of Haematology, Wellcome-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK
Cell Research, 2021-08-02
Abstract

Limited knowledge of cellular and molecular mechanisms underlying hematopoietic stem cell and multipotent progenitor (HSC/MPP) expansion within their native niche has impeded the application of stem cell-based therapies for hematological malignancies.

Here, we constructed a spatiotemporal transcriptome map of mouse fetal liver (FL) as a platform for hypothesis generation and subsequent experimental validation of novel regulatory mechanisms. Single-cell transcriptomics revealed three transcriptionally heterogeneous HSC/MPP subsets, among which a CD93-enriched subset exhibited enhanced stem cell properties. Moreover, by employing integrative analysis of single-cell and spatial transcriptomics, we identified novel HSC/MPP 'pocket-like' units (HSC PLUS), composed of niche cells (hepatoblasts, stromal cells, endothelial cells, and macrophages) and enriched with growth factors.

Unexpectedly, macrophages showed an 11-fold enrichment in the HSC PLUS. Functionally, macrophage–HSC/MPP co-culture assay and candidate molecule testing, respectively, validated the supportive role of macrophages and growth factors (MDK, PTN, and IGFBP5) in HSC/MPP expansion.

Finally, cross-species analysis and functional validation showed conserved cell–cell interactions and expansion mechanisms but divergent transcriptome signatures between mouse and human FL HSCs/MPPs. Taken together, these results provide an essential resource for understanding HSC/MPP development in FL, and novel insight into functional HSC/MPP expansion ex vivo.
Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics_1
Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics_2
Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics_3
Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics_4
  • Perspectives and challenges for lead-free energy-storage multilayer ceramic capacitors
  • Peiyao Zhao, Ziming Cai, Longwen Wu, Chaoqiong Zhu, Longtu Li, Xiaohui Wang
  • Journal of Advanced Ceramics
  • 2021-11-12
  • On the Asymptotic Stability of Wave Equations Coupled by Velocities of Anti-symmetric Type
  • Yan Cui, Zhiqiang Wang
  • Chinese Annals of Mathematics, Series B
  • 2021-11-12
  • Effects on the emission discrepancy between two-dimensional Sn-based and Pb-based perovskites
  • Zhang Tianju, Zhou Chaocheng, Lin Jia, Wang Jun
  • Chinese Optics Letters
  • 2021-11-11
  • Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms
  • Hai-Yang Wu, Lei Yang, Jiang-Shan Tu, Jie Wang, Jin-Ge Li, Hong-Ying Lv, Xiao-Niu Yang
  • Chinese Journal of Polymer Science
  • 2021-11-09
  • Feature and Tendency of Technology Transfer in Z-Park Patent Cooperation Network: From the Perspective of Global Optimal Path
  • Jun Guan, Jingying Xu, Yu Han, Dawei Wang, Lizhi Xing
  • Journal of Data and Information Science
  • 2021-11-08
  • Mechanical Design and Dynamic Compliance Control of Lightweight Manipulator
  • Shao-Lin Zhang, Yue-Guang Ge, Hai-Tao Wang, Shuo Wang
  • International Journal of Automation and Computing
  • 2021-11-06
  • Scalping or Line-sitting: The Role of Speculators
  • Qianqian Chen, Zhenyang Shi, Yi Yang
  • Journal of Systems Science and Systems Engineering
  • 2021-11-06
  • Substrate damage and recovery after giant clam shell mining at remote coral reefs in the southern South China Sea
  • Shengnan Zhou, Qi Shi, Hongqiang Yang, Xiyang Zhang, Xiaoju Liu, Fei Tan, Pin Yan
  • Journal of Geographical Sciences
  • 2021-11-05
  • Some Questions Regarding Verification of Carleson Measures
  • Fangwen Deng, Caiheng Ouyang, Ru Peng
  • Acta Mathematica Scientia
  • 2021-11-05
  • Direct growth of hexagonal boron nitride films on dielectric sapphire substrates by pulsed laser deposition for optoelectronic applications
  • Gaokai Wang, Jingren Chen, Junhua Meng, Zhigang Yin, Ji Jiang, Yan Tian, Jingzhen Li, Jinliang Wu, Peng Jin, Xingwang Zhang
  • Fundamental Research
  • 2021-11-05
  • Climate Suitability Assessment of Human Settlements for Regions along the Belt and Road
  • LIN Yumei, LI Peng, FENG Zhiming, YANG Yanzhao, YOU Zhen, ZHU Fuxin
  • Chinese Geographical Science
  • 2021-11-03



  • Agency and Autonomy in the Maritime Silk Road Initiative: An Examination of Djibouti's Doraleh Container Terminal Disputes                                Identification of twist-angle-dependent excitons in WS₂/WSe₂ heterobilayers
    About
    |
    Contact
    |
    Copyright © PubCard