Year
Month
(Peer-Reviewed) Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome
Yuran Huang 黄宇然 ¹, Zhimin Zhang 张智敏 ¹ ², Wenli Tao 陶汶理 ¹, Yunfei Wei 魏云飞 ³, Liang Xu 徐良 ¹, Wenwen Gong 宫文文 ¹, Jiaqiang Zhou 周嘉强 ⁴, Liangcai Cao 曹良才 ⁵, Yong Liu 刘勇 ⁶, Yubing Han 韩于冰 ¹ ³, Cuifang Kuang 匡翠方 ¹ ² ⁷, Xu Liu 刘旭 ¹ ²
¹ State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, China
中国 杭州 浙江大学光电科学与工程学院 极端光学技术与仪器全国重点实验室
² Research Center for Intelligent Chips and Devices, Zhejiang Lab, Hangzhou 311121, China
中国 杭州 之江实验室 智能芯片与器件研究中心
³ Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
中国 武汉 华中科技大学武汉光电国家研究中心
⁴ Department of Endocrinology and Metabolism, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China
中国 杭州 浙江大学医学院附属邵逸夫医院 内分泌代谢科
⁵ Department of Precision Instruments, Tsinghua University, Beijing 100084, China
中国 北京 清华大学精密仪器系
⁶ College of electronics and information engineering, Shanghai University of Electrical Power, Shanghai 200090, China
中国 上海 上海电力大学 电子与信息工程学院
⁷ ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China
中国 杭州 浙江大学杭州国际科创中心
Opto-Electronic Advances, 2024-07-05
Abstract

Stimulated emission depletion microscopy (STED) holds great potential in biological science applications, especially in studying nanoscale subcellular structures. However, multi-color STED imaging in live-cell remains challenging due to the limited excitation wavelengths and large amount of laser radiation. Here, we develop a multiplexed live-cell STED method to observe more structures simultaneously with limited photo-bleaching and photo-cytotoxicity.

By separating live-cell fluorescent probes with similar spectral properties using phasor analysis, our method enables five-color live-cell STED imaging and reveals long-term interactions between different subcellular structures. The results here provide an avenue for understanding the complex and delicate interactome of subcellular structures in live-cell.
Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome_1
Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome_2
Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome_3
  • Tunable compound eyes with coaxial lens-on-lens ommatidia for cooperative bi-focal imaging
  • Zhi-Juan Sun, Wei-Jian Zhong, Qing Cai, Yi-Fan Lu, Chang-Xu Li, Dong-Dong Han, Yong-Lai Zhang
  • Opto-Electronic Advances
  • 2026-02-09
  • High-efficiency infrared upconversion imaging with nonlinear silicon metasurfaces empowered by quasi-bound states in the continuum
  • Tingting Liu, Jumin Qiu, Meibao Qin, Xu Tu Huifu Qiu, Feng Wu, Tianbao Yu, Qiegen Liu, Shuyuan Xiao
  • Opto-Electronic Advances
  • 2026-01-29
  • Timeshare surface-enhanced Raman scattering platform with sensitive and quantitative mode
  • Qianqian Ding, Xueyan Chen, Yunlu Jia, Hong Liu, Xiaochen Zhang, Ningtao Cheng, Shikuan Yang
  • Opto-Electronic Advances
  • 2026-01-27
  • Electric-field-induced second-harmonic generation
  • Hangkai Fan, Alexey Proskurin, Mingzhao Song, Andrey Bogdanov
  • Opto-Electronic Advances
  • 2026-01-27
  • Fiber-optic microstructured sensors based on abrupt field patterns: theory, fabrication, and applications
  • Yuxuan Yi, Wanlai Zhu, Zao Yi, Zigang Zhou, Shubo Cheng, Majid Niaz Akhtar, Sohail Ahmad
  • Opto-Electronic Science
  • 2026-01-23
  • Integrated metasurface-freeform system enabled multi-focal planes augmented reality display
  • Shifei Zhang, Lina Gao, Yidan Zhao, Yongdong Wang, Bo Wang, Junjie Li, Jiaxi Duan, Dewen Cheng, Cheng-Wei Qiu, Yongtian Wang, Tong Yang, Lingling Huang
  • Opto-Electronic Science
  • 2026-01-23
  • Decoding subject-invariant emotional information from cardiac signals detected by photonic sensing system
  • Yukun Long, Rui Min Kun Xiao, Zhuo Wang, Lanfang Liu, Yifan Sun, Xiaoli Li, Zhaohui Li, Zeev Zalevsky
  • Opto-Electronic Technology
  • 2025-12-25
  • Integrated photonic synapses, neurons, memristors, and neural networks for photonic neuromorphic computing
  • Shufei Han, Weihong Shen, Min Gu, Qiming Zhang
  • Opto-Electronic Technology
  • 2025-12-25
  • Photoacoustic spectroscopy and light-induced thermoelastic spectroscopy based on inverted-triangular lithium niobate tuning fork
  • Junjie Mu, Guowei Han, Runqiu Wang, Shunda Qiao, Ying He Yufei Ma
  • Opto-Electronic Science
  • 2025-12-25
  • Thin-film lithium niobate-based detector: recent advances and perspectives
  • Xiaoli Sun, Yuechen Jia, Feng Chen
  • Opto-Electronic Science
  • 2025-12-25
  • In-situ and ex-situ twisted bilayer liquid crystal computing platform for reconfigurable image processing
  • Kang Zeng, Yougang Ke, Zhangming Hong, Linzhou Zeng, Xinxing Zhou
  • Opto-Electronic Advances
  • 2025-12-25
  • Highly textured single-crystal-like perovskite films for large-area, high-performance photodiodes
  • Runkai Liu, Feng Li, Rongkun Zheng
  • Opto-Electronic Advances
  • 2025-12-25



  • Reconfigurable optical neural networks with Plug-and-Play metasurfaces                                OptoGPT: A foundation model for inverse design in optical multilayer thin film structures
    About
    |
    Contact
    |
    Copyright © PubCard