(Peer-Reviewed) Discovery of potential anti-SARS-CoV-2 drugs based on large-scale screening in vitro and effect evaluation in vivo
Haoran Peng 彭浩然 ¹, Cuiling Ding 丁翠玲 ¹, Liangliang Jiang 江亮亮 ¹, Wanda Tang ¹, Yan Liu 刘燕 ¹, Lanjuan Zhao 赵兰娟 ¹, Zhigang Yi 易志刚 ², Hao Ren 任浩 ¹, Chong Li ³, Yanhua He 何燕华 ¹, Xu Zheng ¹, Hailin Tang 唐海琳 ¹, Zhihui Chen 陈志辉 ⁴, Zhongtian Qi 戚中田 ¹, Ping Zhao 赵平 ¹
¹ Department of Microbiology, Second Military Medical University, Shanghai Key Laboratory of Medical Biodefense, Shanghai, 200433, China
中国 上海 第二军医大学微生物学教研室 上海市医学生物防护重点实验室
² Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China
中国 上海 复旦大学上海医学院基础医学院 医学分子病毒学教育部/卫生部/医科院重点实验室
³ Cancer Institute, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200000, China
中国 上海 复旦大学上海医学院肿瘤学系 复旦大学附属肿瘤医院 肿瘤研究所
⁴ Department of Infectious Disease, Changhai Hospital, Shanghai, 200433, China
中国 上海 长海医院感染科

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global crisis. Clinical candidates with high efficacy, ready availability, and that do not develop resistance are in urgent need. Despite that screening to repurpose clinically approved drugs has provided a variety of hits shown to be effective against SARS-CoV-2 infection in cell culture, there are few confirmed antiviral candidates in vivo.

In this study, 94 compounds showing high antiviral activity against SARS-CoV-2 in Vero E6 cells were identified from 2,580 FDA-approved small-molecule drugs. Among them, 24 compounds with low cytotoxicity were selected, and of these, 17 compounds also effectively suppressed SARS-CoV-2 infection in HeLa cells transduced with human ACE2. Six compounds disturb multiple processes of the SARS-CoV-2 life cycle. Their prophylactic efficacies were determined in vivo using Syrian hamsters challenged with SARS-CoV-2 infection. Seven compounds reduced weight loss and promoted weight regain of hamsters infected not only with the original strain but also the D614G variant.

Except for cisatracurium, six compounds reduced hamster pulmonary viral load, and IL-6 and TNF-α mRNA when assayed at 4 d postinfection. In particular, sertraline, salinomycin, and gilteritinib showed similar protective effects as remdesivir in vivo and did not induce antiviral drug resistance after 10 serial passages of SARS-CoV-2 in vitro, suggesting promising application for COVID-19 treatment.
Discovery of potential anti-SARS-CoV-2 drugs based on large-scale screening in vitro and effect evaluation in vivo_1
Discovery of potential anti-SARS-CoV-2 drugs based on large-scale screening in vitro and effect evaluation in vivo_2
Discovery of potential anti-SARS-CoV-2 drugs based on large-scale screening in vitro and effect evaluation in vivo_3
Discovery of potential anti-SARS-CoV-2 drugs based on large-scale screening in vitro and effect evaluation in vivo_4
  • Preplanned Studies: Disease Burden of Pancreatic Cancer — China, 1990−2019
  • Yuan He, Xiaolong Zhou, Xueqi Fan, Bin Zhang, Li Ma, Jing Wu, Xudong Li
  • China CDC Weekly
  • 2022-03-23
  • Effects of low molecular weight organic acids on aggregation behavior of biochar colloids at acid and neutral conditions
  • Yang Wang, Changxi Wang, Jiayi Xiong, Qianru Zhang, Jianying Shang
  • Biochar
  • 2022-03-23
  • A universal spray printing strategy to prepare gradient hybrid architectures
  • Lingyu Du, Songshan Bi, Yang Hu, Rui Wang, Jiacai Zhu, Minghui Zhang, Zhiqiang Niu
  • Carbon Energy
  • 2022-03-21
  • Terahertz metasurface zone plates with arbitrary polarizations to a fixed polarization conversion
  • Zhen Yue, Jitao Li 李继涛, Jie Li, Chenglong Zheng, Jingyu Liu, Guocui Wang, Hang Xu, Mingyang Chen, Yating Zhang, Yan Zhang, Jianquan Yao
  • Opto-Electronic Science
  • 2022-03-18
  • Single-shot mid-infrared incoherent holography using Lucy-Richardson-Rosen algorithm
  • Vijayakumar Anand, Molong Han, Jovan Maksimovic, Soon Hock Ng, Tomas Katkus, Annaleise Klein, Keith Bambery, Mark J. Tobin, Jitraporn Vongsvivut, Saulius Juodkazis
  • Opto-Electronic Science
  • 2022-03-18
  • A virus-derived small RNA targets the rice transcription factor ROC1 to induce disease-like symptom
  • Xiuqin Huang, Feifei Li, Xishan Zhang, Jiahao Chen, Junkai Wang, Jing Wei, Xin Yang, Guohui Zhou, Tong Zhang
  • Phytopathology Research
  • 2022-03-17
  • Effects of β-carotene on glucose metabolism dysfunction in human subjects and type 2 diabetic rats
  • Jianjun Wu, Yinan Zhou, Hanqing Hu, Dawei Yang, Fan Yang
  • Deep Science
  • 2022-03-17
  • Advances in phase-sensitive optical time-domain reflectometry
  • Shuaiqi Liu, Feihong Yu, Rui Hong, Weijie Xu, Liyang Shao, Feng Wang
  • Opto-Electronic Advances
  • 2022-03-15
  • Second harmonic generation of laser beams in transverse mode locking states
  • Zilong Zhang, Yuan Gao, Xiangjia Li, Xin Wang, Suyi Zhao, Qiang Liu, Changming Zhao
  • Advanced Photonics
  • 2022-03-14
  • A combined multiscale modeling and experimental study on surface modification of high-volume micro-nanoparticles with atomic accuracy
  • Zoushuang Li, Junren Xiang, Xiao Liu, Xiaobo Li, Lijie Li, Bin Shan, Rong Chen
  • International Journal of Extreme Manufacturing
  • 2022-03-09
  • Tailoring a novel hierarchical cheese-like porous biochar from algae residue to boost sulfathiazole removal
  • Ke Wang, Yue Wang, Shiyu Zhang, Yi-di Chen, Rupeng Wang, Shih-Hsin Ho
  • Environmental Science & Ecotechnology
  • 2022-03-09
  • Giant magneto field effect in up-conversion amplified spontaneous emission via spatially extended states in organic-inorganic hybrid perovskites
  • Tangyao Shen, Jiajun Qin, Yujie Bai, Jia Zhang, Lei Shi, Xiaoyuan Hou, Jian Zi, Bin Hu
  • Opto-Electronic Advances
  • 2022-02-28

  • Directional sliding of water: biomimetic snake scale surfaces                                Time-dependent borehole stability in hard-brittle shale
    Copyright © PubCard